Verify that your standard curve has the log of DNA copies on the x-axis and Ct on the y-axis.
1. Find the slope of your standard dilution curve (e.g., -3.35).
2. Insert the slope value into the field below.
3. The calculator will determine the qPCR efficiency and amplification factor.

If the efficiency is between 90% and 110%, it's acceptable. If it's outside this range, consider optimizing your PCR conditions.